Items

There are 36 annotated items in total.

Annotated References

Alekseenko, A., Barrett, D., Pareja-Sanchez, Y., Howard, R. J., Strandback, E., Ampah-Korsah, H., Rovšnik, U., Zuniga-Veliz, S., Klenov, A., Malloo, J., Ye, S., Liu, X., Reinius, B., Elsässer, S. J., Nyman, T., Sandh, G., Yin, X., & Pelechano, V. (2021). Direct detection of SARS-CoV-2 using non-commercial RT-LAMP reagents on heat-inactivated samples. Scientific reports, 11(1), 1820. https://doi.org/10.1038/s41598-020-80352-8

• STARS: 2

• ANNOTE

  • RT-LAMP detects medium-high viral loads in swabs without RNA extraction and purification

  • Describes homebrew production of B.stearothermophilus Bst-LF ‘v5.9’ from Ellington et al

  • Provides an alternative to commercial reagents for RT-LAMP based SARS-COv2 detection.

Becherer, L N. Borst, M. Bakheit, S. Frischmann, R. Zengerle, and F. von Stetten, “Loop-mediated isothermal amplification (LAMP) – review and classification of methods for sequence-specific detection,” Analytical Methods, vol. 12, no. 6, pp. 717–746, 2020.

• STARS: 2

• ANNOTE

  • LAMP has a propensity to create non-specific amplicons caused by mis-priming

  • Sequence-specific amplicon detection can significantly increase LAMP specificity

  • The Review provides a systematic, comprehensive summary to the analytical options

  • Several of the fluorescent methods described have been applied to SARS-CoV2 LAMP

  • We foresee an ever-increasing proportion of multiplexed, multicolour LAMP tests

Ben-Assa, N et al. , “Direct on-the-spot detection of SARS-CoV-2 in patients,” Experimental Biology and Medicine; , vol. 245, no. 14, pp. 1187–1193, jul 2020.

• STARS: 1

• ANNOTE

  • An early report of the use of saliva (cf. UTM/VTM) in pH-based colourimetric RT-LAMP

  • Simplified sample processing with proteinase K prior to heating and the RT-LAMP test

  • Clinical testing yielded acceptable sensitivity equivalent to RT-qPCR cut-off Ct ≲ 29

  • Provides a good example of method development and initial clinical validation

Bektas, A., Covington, M., Aidelberg, G., Arce, A., Matute, T., N\'u\~nez, I., Walsh, J., Boutboul, D., Lindner, A., Federici, F., & Jayaprakash, A. (2021). Accessible LAMP-Enabled Rapid Test (ALERT) for detecting SARS-CoV-2. medRxiv. https://doi.org/10.3390/v13050742

• STARS: 3

• ANNOTE

  • ALERT: a streamlined process that improves overall LAMP performance and usability

  • Separating RT reaction from LAMP significantly improves limit of detection

  • Sequence-specific QUASR probe improves specificity, reducing false positive rates

  • Co-detection with other respiratory viruses by multiplexing with QUASR fluorescent probes

  • Use of wax layers to lengthen reagent shelf-life of ready-to-use mixes at room temperature

Bhadra, S., Riedel, T., Lakhotia,S., Tran, N.D., and Ellington, A. High-surety isothermal amplification and detection of SARS-CoV-2, including with crude enzymes bioRxiv 2020.04.13.039941; doi: https://doi.org/10.1101/2020.04.13.039941 ; Now accepted for publication in mSphere

• STARS: 3

• ANNOTE

  • Converts three assays using non-specific to a sequence-specific fluorescence readout

  • One-strand displacement (OSD) creates a stable, multicolor readout for saliva LAMP

  • One-pot reactions assembled using ‘homebrew’ enzyme reagents

  • Highly versatile multiplex method well-suited to primary screening in resource limited settings

  • One of a core of ~ 5-6 sequence-specific methods which we foresee to increase in use

Broughton, J et al. , “CRISPR–cas12-based detection of SARS-CoV-2,” Nature Biotechnology , vol. 38, no. 7, pp. 870–874, apr 2020.

• STARS: 1

• ANNOTE

  • The CRISPR-Cas system detects E and N gene sequences by using specific guide RNA

  • Once bound to cDNA amplified by RT-LAMP, collateral Cas12 ssDNase activity is activated

  • The ssDNase activity can be readily monitored by quenched fluorescence or lateral flow

  • With a LoD ~ 10 copies/uL, the DETECTR system demonstrated 95% sensitivity/100% specificity

  • CRISPR has potential to increase specificity and sensitivity albeit at the cost of complexity

Corman, V. M., Landt, O., Kaiser, M., Molenkamp, R., Meijer, A., Chu, D. K., Bleicker, T., Brünink, S., Schneider, J., Schmidt, M. L., Mulders, D. G., Haagmans, B. L., van der Veer, B., van den Brink, S., Wijsman, L., Goderski, G., Romette, J. L., Ellis, J., Zambon, M., Peiris, M., … Drosten, C. (2020). Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR. Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin, 25(3), 2000045. https://doi.org/10.2807/1560-7917.ES.2020.25.3.2000045

• STARS: 2

• ANNOTE

  • Early reference paper, endorsed by the WHO, on which many RT-qPCR tests were based

  • Created a workflow for detecting SARS-CoV-2 vs. other human respiratory viruses

  • RNA controls are made available through European Virus Archive (EVAg)

  • Research and RNA controls made possible by widespread coordination across the EU.

de Oliveira Coelho, B., Sanchuki, H., Zanette, D. L., Nardin, J. M., Morales, H., Fornazari, B., Aoki, M. N., & Blanes, L. (2021). Essential properties and pitfalls of colorimetric Reverse Transcription Loop-mediated Isothermal Amplification as a point-of-care test for SARS-CoV-2 diagnosis. Molecular medicine (Cambridge, Mass.), 27(1), 30. https://doi.org/10.1186/s10020-021-00289-0

• STARS: 2

• ANNOTE

  • Diagnostic comparison (94% sensitivity, 90% specificity) using RNA extracted from NPS

  • Highlights an intermediate orange result when using a pH sensitive readout (red or yellow)

  • Orange readout is characteristic of samples with corresponding RT-qPCR ≥ 30 Ct.

  • Insight into a range of low/indeterminate viral load where confirmatory testing is required.

Ding, Sheng, et al. “Sequence-Specific and Multiplex Detection of COVID-19 Virus (SARS-CoV-2) Using Proofreading Enzyme-Mediated Probe Cleavage Coupled with Isothermal Amplification.” Biosensors & Bioelectronics, vol. 178, Apr. 2021, p. 113041. https://doi.org/10.1016/j.bios.2021.113041.

• STARS: 1

• ANNOTE

  • Addresses the false positives arising from lack of sequence-specificity in many LAMP readouts

  • Uses Pfu proof-reading enzyme-mediated probe cleavage (RT-Proofman-LAMP)

  • Real-time or end-point monitoring in a closed-tube format suitable for point-of-care testing

  • Sequence-specificity enables multiplexing COVID-19 genes and internal control amplicons

  • Recent addition to the list of multiplexed, sequence specific detection methods for LAMP

Maranhao, Andre, et al. An Improved and Readily Available Version of Bst DNA Polymerase for LAMP, and Applications to COVID-19 Diagnostics. preprint, Infectious Diseases (except HIV/AIDS), 5 Oct. 2020. https://doi.org/10.1101/2020.10.02.20203356.

• STARS: 1

• ANNOTE

  • Protein engineering fusion improves function of Bst DNA polymerase in LAMP

  • Modifying Bst DNA polymerase significantly improves reverse transcriptase activity

  • Further improves LAMP-OSD detection in pre-heated saliva without RNA extraction

Fowler, V. L., Armson, B., Gonzales, J. L., Wise, E. L., Howson, E., Vincent-Mistiaen, Z., Fouch, S., Maltby, C. J., Grippon, S., Munro, S., Jones, L., Holmes, T., Tillyer, C., Elwell, J., Sowood, A., de Peyer, O., Dixon, S., Hatcher, T., Patrick, H., Laxman, S., … Kidd, S. P. (2021). A highly effective reverse-transcription loop-mediated isothermal amplification (RT-LAMP) assay for the rapid detection of SARS-CoV-2 infection. The Journal of infection, 82(1), 117–125. https://doi.org/10.1016/j.jinf.2020.10.039

• STARS: 2

• ANNOTE

  • Reports a novel RT-LAMP assay with 97% and 99% clinical sensitivity and specificity, respectively

  • Almost perfect agreement with RNA RT-LAMP (extracted RNA) and RT-qPCR

  • Proposes replacing RT-qPCR with RNA RT-LAMP for higher throughput

  • Suggests using direct RT-LAMP (1:20 VTM dilution) for screening the highly infectious

Ganguli, A., Mostafa, A., Berger, J., Aydin, M., Sun, F., Valera, E., Cunningham, B. T., King, W. P., & Bashir, R. (2020). Rapid Isothermal Amplification and Portable Detection System for SARS-CoV-2. bioRxiv : the preprint server for biology, 2020.05.21.108381. https://doi.org/10.1101/2020.05.21.108381

• STARS: 1

• ANNOTE

  • Introduces an RNA extraction-free method from patient swab samples via heat-lysis

  • Microfluidic-phone camera example of portable hardware system for biosurveillance

Garneret, P., Coz, E., Martin, E., Manuguerra, J. C., Brient-Litzler, E., Enouf, V., González Obando, D. F., Olivo-Marin, J. C., Monti, F., van der Werf, S., Vanhomwegen, J., & Tabeling, P. (2021). Performing point-of-care molecular testing for SARS-CoV-2 with RNA extraction and isothermal amplification. PloS one, 16(1), e0243712. https://doi.org/10.1371/journal.pone.0243712

• STARS: 1

• ANNOTE

  • COVIDISC, a paper-based fabricated portable device for point-of-care testing

  • Combines RNA extraction and running RT-LAMP reactions in one device

  • With VTM, comparable analytical sensitivity and specificity vs RT-qPCR

  • Cost-effective at $2-4 per device, removing the need for complex instruments.

James, P., Stoddart, D., Harrington, E., Beaulaurier, J., Ly, L., Reid, S., Turner, D., & Juul, S. (2020). LamPORE: rapid, accurate and highly scalable molecular screening for SARS-CoV-2 infection, based on nanopore sequencing. medRxiv.

• STARS: 2

• ANNOTE

  • LAMPoreTM combines triplexed SARS-CoV-2 detection and nanopore DNA sequencing

  • Performs 96 reactions in two hours with good concordance with RT-qPCR using swab samples

  • Example of the potential for integrating sequencing with LAMP e.g for variant detection.

Jureka, A. S., Silvas, J. A., & Basler, C. F. (2020). Propagation, Inactivation, and Safety Testing of SARS-CoV-2. Viruses, 12(6), 622. https://doi.org/10.3390/v12060622

• STARS: 1

• ANNOTE

  • Important methods and considerations for live viral manipulation/inactivation

  • Propagation of SARS-CoV-2 in different cell lines to augment supply of control RNA

  • Lists various viral Inactivation methods to allow research be done at lower biosafety levels

Kahl, L., Molloy, J., Patron, N. et al. Opening options for material transfer. Nat Biotechnol 36, 923–927 (2018). https://doi.org/10.1038/nbt.4263

• STARS: 2

• ANNOTE

  • Introduces the principles of Open Material Transfer Agreements in the open bioeconomy

  • Relaxes the outdated restrictions on redistribution and commercial use of biological materials

  • Better adapted to current research practices allowing broder sharing of materials and technology

Kellner, M., Ross, J., Schnabl, J., Dekens, M., Heinen, R., Grishkovskaya, I., Bauer, B., Stadlmann, J., Menéndez-Arias, L., Fritsche-Polanz, R., Traugott, M., Seitz, T., Zoufaly, A., Födinger, M., Wenisch, C., Zuber, J., , Pauli, A., & Brennecke, J. (2020). A rapid, highly sensitive and open-access SARS-CoV-2 detection assay for laboratory and home testing. bioRxiv.

• STARS: 3

• ANNOTE

  • Introduces an extraction-free pH buffered Hydroxynapthol blue (HNB)-based RT-LAMP

  • Highlights the use of the dUTP/UDG system to prevent carryover contamination

  • Improves LAMP sensitivity through RNA purification via use of magnetic beads

  • Compares commercial and homebrew LAMP enzymes to develop lower-cost test

  • A comprehensive and highly cited paper from the influential Vienna group

Larremore, D., Wilder, B., Lester, E., Shehata, S., Burke, J., Hay, J., Tambe, M., Mina, M., & Parker, R. (2020). Test sensitivity is secondary to frequency and turnaround time for COVID-19 surveillance. medRxiv.

• STARS: 2

• ANNOTE

  • Challenges the assumption that clinical sensitivity is paramount for effective surveillance

  • Highlights the importance of testing frequency and reporting speed

  • High frequency and large scale testing is more effective than high sensitivity

  • Clinical sensitivity cannot be simply be defined by the limit of analytical detection

  • Provides rationale for widespread use of LAMP with more rapid reporting than qPCR.

Mina, M. J., Parker, R., & Larremore, D. B. (2020). Rethinking Covid-19 Test Sensitivity - A Strategy for Containment. The New England journal of medicine, 383(22), e120. https://doi.org/10.1056/NEJMp2025631

• STARS: 3

• ANNOTE

  • Reframes and challenges the current predominant view on COVID-19 testing sensitivity

  • Highlights that analytical sensitivity and clinical sensitivity are not the same

  • Recommends that clinical sensitivity be contextualized and defined as fit-for-purpose

Moehling, T. J., Choi, G., Dugan, L. C., Salit, M., & Meagher, R. J. (2021). LAMP Diagnostics at the Point-of-Care: Emerging Trends and Perspectives for the Developer Community. Expert review of molecular diagnostics, 21(1), 43–61. https://doi.org/10.1080/14737159.2021.1873769

• STARS: 1

• ANNOTE

  • Complementary review providing valuable insights on development of LAMP diagnostics

  • Describes advantages and disadvantages of different human samples

  • Highlights mispriming in saliva-based LAMP as a source of false positives

  • Emphasizes the need for sequence-specific detection, e.g QUASR, to increase specificity.

Natoli, M., Kundrod, K., Chang, M., Smith, C., Paul, S., Eldin, K., Patel, K., Baker, E., Schmeler, K., & Richards-Kortum, R. (2021). Reverse transcription loop-mediated isothermal amplification (Rt-lamp) for point-of-care detection of SARS-CoV-2: A clinical study to evaluate agreement with RT-qPCR. The Lancet. Global Health, 9, S3. https://doi.org/10.1016/S2214-109X(21)00111-X

• STARS: 1

• ANNOTE

  • Evaluates the clinical performance of NPS-based but extraction-free RT-LAMP test

  • Performance (92% sensitivity, 91% specificity) was maintained using a lower-cost reader

  • An example of many such studies reporting comparable performance of LAMP vs qPCR

Notomi, T., Okayama, H., Masubuchi, H., Yonekawa, T., Watanabe, K., Amino, N., & Hase, T. (2000). Loop-mediated isothermal amplification of DNA. Nucleic acids research, 28(12), E63. https://doi.org/10.1093/nar/28.12.e63

• STARS: 3

• ANNOTE

  • Pioneer LAMP paper: introduces LAMP as a novel DNA amplification method

  • Highly specific and sensitive isothermal amplification with a strand-displacing polymerase

  • Initial paper describes use of 4 primers; later extended to include 2 Loop primers

  • Methodology is operationally simple but mechanistically and bioinformatically complex

Rabe, B. A., & Cepko, C. (2020). SARS-CoV-2 detection using isothermal amplification and a rapid, inexpensive protocol for sample inactivation and purification. Proceedings of the National Academy of Sciences of the United States of America, 117(39), 24450–24458. https://doi.org/10.1073/pnas.2011221117

• STARS: 3

• ANNOTE

  • Pivotal paper for low-cost RNA extraction-free methods from human samples

  • A basis for many succeeding papers that uses heat/TCEP/EDTA to inactivate RNases

  • Bead-based and extraction-free (‘direct’) RT-LAMP methods are described in detail.

  • Demonstrates that NaI-based inactivation and purification system improves LAMP sensitivity

Sherrill-Mix, S., Duyne, G., & Bushman, F. (2021a). Molecular beacons allow specific RT-LAMP detection of B.1.1.7 variant SARS-CoV-2. medRxiv.

• STARS: 2

• ANNOTE

  • Demonstrates the ability of hybridisation-based molecular beacons to detect variants

  • Pilot study on variant-discrimination for SARS-CoV-2 (S1∆69-70 deletion)

  • Targeted approach that aids in managing and containing the spread of the COVID-19 variants

Sherrill-Mix, S., Hwang, Y., Roche, A., Glascock, A., Weiss, S., Li, Y., Haddad, L., Deraska, P., Monahan, C., Kromer, A., Graham-Wooten, J., Taylor, L., Abella, B., Ganguly, A., Collman, R., Van Duyne, G., & Bushman, F. (2021b). LAMP-BEAC: Detection of SARS-CoV-2 RNA Using RT-LAMP and Molecular Beacons. medRxiv.

• STARS: 3

• ANNOTE

  • Excellent example of use of molecular beacons for sequence-specific amplicon detection

  • Addresses the false positives arising LAMP to improve reliability and sensitivity of a test

  • Unique and characteristic melt curves provide additional level of specificity

  • Demonstrated multiplexed detection with extraction-free saliva RT-LAMP.

Snodgrass, R., Gardner, A., Semeere, A., Kopparthy, V. L., Duru, J., Maurer, T., Martin, J., Cesarman, E., & Erickson, D. (2018). A portable device for nucleic acid quantification powered by sunlight, a flame or electricity. Nature biomedical engineering, 2(9), 657–665. https://doi.org/10.1038/s41551-018-0286-y

• STARS: 1

• ANNOTE

  • Addresses poor diagnostic infrastructure due unreliable access to electricity in Africa

  • TINY: portable microfluidic LAMP device, operating with electricity and alternative power sources

  • 94% agreement between TINY and commercial PCR for the detection of herpesvirus

  • Example of small, simple devices for PCR that can be applied to LAMP.

Tan, S. H.; Allicock, O.; Armstrong-Hough, M. & Wyllie, A. L. Saliva as a gold-standard sample for SARS-CoV-2 detection The Lancet Respiratory Medicine, Elsevier BV, 2021, 9, 562-564 https://doi.org/10.1016/s2213-2600(21)00178-8

• STARS: 3

• ANNOTE

  • Mini-review summarising the potential and pitfalls of using saliva as a biospecimen

  • Focus is on collection, handling, transport, and storage of saliva relative to NPS

  • Comprehensive Supplementary Information tabulating large number of comparative studies

  • Discusses points of disagreement regarding performance of saliva- vs NPS-based tests

  • A balanced minireview but with clear advocacy for saliva if carefully collected and processed.

Tanner, N. A., Zhang, Y., & Evans, T. C. (2012). Simultaneous multiple target detection in real-time loop-mediated isothermal amplification. BioTechniques, 53(2), 81–89. https://doi.org/10.2144/0000113902

• STARS: 1

• ANNOTE

  • First report of ‘DARQ’ fluorescent LAMP for multiplexed detection of four targets

  • Initial reports of the widely-used Bst 2.0 and WarmStart Bst 2.0 DNA polymerases

  • Bst 2.0 speeds up the LAMP reaction signal by 50% over wild-type Bst DNA polymerase

Vogels, C., Watkins, A. E., Harden, C. A., Brackney, D. E., Shafer, J., Wang, J., Caraballo, C., Kalinich, C. C., Ott, I. M., Fauver, J. R., Kudo, E., Lu, P., Venkataraman, A., Tokuyama, M., Moore, A. J., Muenker, M. C., Casanovas-Massana, A., Fournier, J., Bermejo, S., Campbell, M., … Grubaugh, N. D. (2021). SalivaDirect: A simplified and flexible platform to enhance SARS-CoV-2 testing capacity. Med (New York, N.Y.), 2(3), 263–280.e6. https://doi.org/10.1016/j.medj.2020.12.010

• STARS: 2

• ANNOTE

  • First saliva-based SARS-CoV-2 detection to be granted EUA by the FDA

  • Led to increased awareness and interest in saliva as a biospecimen type

  • Using RT-qPCR, SalivaDirect and nasopharyngeal swabs have high positive agreement (94%)

  • Early example of a viable cost-effective alternative method to improve accessibility

Wang, Rui, et al. “OpvCRISPR: One-Pot Visual RT-LAMP-CRISPR Platform for SARS-Cov-2 Detection.” Biosensors and Bioelectronics, vol. 172, Jan. 2021, p. 112766. https://doi.org/10.1016/j.bios.2020.112766.

• STARS: 2

• ANNOTE

  • CRISPR incorporates gRNA for specificity with enzymatic amplification to increase sensitivity.

  • Incorporating CRISPR-Cas12a improves LAMP sensitivity by a factor of 10

  • opvCRISPR combines RT-LAMP and CRISPR-Cas12a with a colorimetric readout in a single well

  • Clinically, 100% positive agreement between RT-qPCR and opvCRISPR

  • Alternative CRISPR methods use Cas13 rather than Cas12.

Wölfel, R., Corman, V.M., Guggemos, W. et al. Virological assessment of hospitalized patients with COVID-2019. Nature 581, 465–469 (2020). https://doi.org/10.1038/s41586-020-2196-x

• STARS: 2

• ANNOTE

  • Early report monitoring the viral load, replication rates and clinical symptoms from nine COVID cases

  • Respiratory samples with < 10^6 copies per mL did not yield a replicative isolate

  • Discharge is proposed for patients beyond day 10 or with < 100000 viral RNA copies / mL.

  • Both criteria predict low residual risk of infectivity based on cell culture.

  • Analytical LoD of RT-qPCR far exceeds the clinically relevant viral loads for many patients

  • Provides data to determine what is a functionally important sensitivity for LAMP tests.

Yan, C., et al. “Rapid and Visual Detection of 2019 Novel Coronavirus (SARS-CoV-2) by a Reverse Transcription Loop-Mediated Isothermal Amplification Assay.” Clinical Microbiology and Infection, vol. 26, no. 6, June 2020, pp. 773–79. https://doi.org/10.1016/j.cmi.2020.04.001.

• STARS: 2

• ANNOTE

  • Thorough description of primer design strategy to create primer sets for orf1ab and S genes.

  • First to demonstrate 100% sensitivity and 100% specificity on clinical samples via visual readout

  • The LAMP assay discriminates SARS-CoV-2 from 60 other respiratory pathogens

  • Highlights value of having multiple primer sets to discriminate related viruses

  • Good example of a design to method development to clinical validation study

Yang, Q., Meyerson, N. R., Clark, S. K., Paige, C. L., Fattor, W. T., Gilchrist, A. R., Barbachano-Guerrero, A., Healy, B. G., Worden-Sapper, E. R., Wu, S. S., Muhlrad, D., Decker, C. J., Saldi, T. K., Lasda, E., Gonzales, P., Fink, M. R., Tat, K. L., Hager, C. R., Davis, J. C., … Sawyer, S. L. (2021). Saliva TwoStep for rapid detection of asymptomatic SARS-CoV-2 carriers. ELife, 10, e65113. https://doi.org/10.7554/eLife.65113.

• STARS: 1

• ANNOTE

  • Saliva-based detection of SARS-CoV-2 in asymptomatic individuals at 94% sensitivity

  • Neutralising saliva stabilisation solution with Proteinase K for saliva-based RT-LAMP

  • Example of addressing need for simple and portable yet reliable test for biosurveillance

Yaren, O., McCarter, J., Phadke, N., Bradley, K. M., Overton, B., Yang, Z., Ranade, S., Patil, K., Bangale, R., & Benner, S. A. (2021). Ultra-rapid detection of SARS-CoV-2 in public workspace environments. PloS one, 16(2), e0240524. https://doi.org/10.1371/journal.pone.0240524

• STARS: 3

• ANNOTE

  • Development of a sequence-specific method for amplicon detection (displaceable probe, DP)

  • Reports successful use of two COVID-19 primer sets with Rnase P and DP in a single tube assay

  • Simplified cost-effective RNA extraction-free method suitable for large scale testing

  • Highlights the value of widespread, frequent testing of asymptomatics - ‘test often, test quickly’.

Zhang, Y., Odiwuor, N., Xiong, J., Sun, L., Nyaruaba, R., Wei, H., & Tanner, N. (2020). Rapid Molecular Detection of SARS-CoV-2 (COVID-19) Virus RNA Using Colorimetric LAMP. medRxiv.

• STARS: 2

• ANNOTE

  • An early paper demonstrating the feasibility of SARS-CoV-2 detection using RT-LAMP

  • Vital study that designed five LAMP primer sets targeting SARS-CoV-2 RNA

  • The primers could reliably detect 120 copies/reaction using colorimetric (pH-based) LAMP

  • Served as foundation for many succeeding (colorimetric) RT-LAMP COVID-19 papers

Zhang, Yinhua, and Nathan A. Tanner. Development of Multiplexed RT-LAMP for Detection of SARS-CoV-2 and Influenza Viral RNA. preprint, Epidemiology, 27 Oct. 2020. https://doi.org/10.1101/2020.10.26.20219972.

• STARS: 3

• ANNOTE

  • Describes application of DARQ for sequence-specific detection of SARS-CoV2 amplicons

  • Multiplexing of SARS-CoV2 and influenza virus as pathogens leading to similar symptoms

  • Related to another sequence-specific detection method (QUASR, Betkas et al., 2021)

  • Enables real-time fluorescence monitoring c.f end-point measurement for QUASR